Positional cloning in maize ( Zea mays subsp. mays , Poaceae)
نویسندگان
چکیده
منابع مشابه
Identification of downy mildew resistance gene candidates by positional cloning in maize (Zea mays subsp. mays; Poaceae)1
PREMISE OF THE STUDY Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates. METHODS AND RESULT...
متن کاملMaize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملLocalization of the genes controlling B chromosome transmission rate in maize (Zea mays ssp. mays, Poaceae).
In previous papers we found that the frequency of B chromosomes in native races of maize varies considerably in different populations. Moreover, we found genotypes that control high and low transmission rates (TR) of B chromosomes in the Pisingallo race. In the present work crosses were made to determine whether the genes controlling B-TR are located on the normal chromosome set (As) or on the ...
متن کاملEpistasis in Maize (zea Mays
ISHER (1918) provided a framework for the characterization of genotypic variance whereby he partitioned it into three components: ( 1 ) a portion associated with the average effects of genes, (2) a portion associated with dominance effects (allelic interactions) of genes, and (3) a portion associated with epistatic effects (nonallelic interactions) of genes. A logical method for the further sub...
متن کاملCloning and expression analysis of hemoglobin genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis).
With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at ide...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2015
ISSN: 2168-0450,2168-0450
DOI: 10.3732/apps.1400092